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The weights of proteins are their corresponding weighted degree.
Proteins are separated by ultracentrifugation very high speed spinning; with appropriate photography of the protein layers as they form in the centrifugal field, it is possible to determine the molecular weights of proteins.
The technique is also a powerful tool for estimating the molecular weights of proteins [25].
The membranes were also subjected to the determination of molecular weight cut-off (MWCO) using different molecular weights of proteins.
The tool "Compute pI/Mw" from ExPASy Proteomics Server (http://www.expasy.org) was used to calculate molecular weights of proteins for their identification on SDS-PAGEs.
Through densitometry analysis, the electrophoretic mobility was calculated, using molecular weights of proteins with reference to protein marker standards (Priya et al. 2013).
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Calibration curve was obtained by plotting the logarithm of known molecular weights of protein versus their respective Ve/V0 value (Ve is the elution volume of the protein and V0 is the void volume of column).
Molecular weights of protein standards are indicated to left.
Molecular marker kit (Bio-Rad) was used for the reference of molecular weights of protein.
The extent of hydration of protein molecules in aqueous solutions is important, because some of the methods used to determine the molecular weight of proteins yield the molecular weight of the hydrated protein.
Although useful for the determination of molecular-weights of proteins, its application to the quantitative determination of protein concentrations is more difficult.
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