They increase the endometrium's size and weight, cell number, cell types, blood flow, protein content, and enzyme activity.
The α-amylase activity in the AmyA-displaying strain was 10.4 ± 1.0 (U/g dry weight cell) at 0 h, but was only 0.1 ± 0.06 (U/g dry weight cell) in the control strain (Table 2).
This was achieved by analyzing E. gracilis physiological parameters, such as cell density, dry weight, cell length, pigments, and paramylon content.
After 10 h of culture, the AmyA-displaying strain had retained its α-amylase activity, which was 9.5 ± 0.5 (U/g dry weight cell).
A week after growth (no apparent adhesive oil residues), 10 20 ml culture was sampled for determination of cell dry weight (cell mass), floating oils and emulsified oils at various time points in triplicate.
Outcome measures were weight loss, wet lung weight, cell count in bronchoalveolar lavage fluid (BALF), and colony-forming units (CFUs) in lung homogenate.
Genomic DNA was prepared from at least 50 mg (wet weight) cell pellets, harvested from mycobacteria that were cultivated on egg-yolk agar media as previously described [ 42].
An OD600 of 1.0 corresponded to 0.39 mg dry weight cells ml-1.
As a result, about 40 g wet weight cells per liter were obtained.
The remaining fresh weight cells were placed in a freezer at −30 °C overnight and then freeze-dried for 3 days.
