The Kidneys and the livers were weighted, snap-frozen immediately in liquid nitrogen, and stored at −80°C until further analysis.
Fractions for the different measurements were weighted into snap cap vials and stored in the fridge until measurement.
Tumor xenografts were harvested, weighted, and snap-frozen.
Liver tissues were dissected on ice and immediately weighted before being snap-frozen in liquid nitrogen.
Livers were excised, weighted, and were either snap frozen or fixed in formalin and paraffin-embedded.
All brains were immediately removed, weighted, the cortex removed, snap-frozen in liquid nitrogen and stored at −80°C for protein extraction.
Twenty-four hours after last treatment (short-term study) or when tumors reached ≥150 mm² (long-term study), respectively, mice were sacrificed, tumors obtained, weighted, bisected, and either snap-frozen for RNA and protein analyses or preserved for histological analyses.
The heart was removed and weighted, and the apex of the left ventricle was snap-frozen in liquid nitrogen for RNA extraction.
The big flag over the Dirksen excavation is snapping in the wind, bright-yellow leaves zipping past as if weighted.
The SNAP-II is a validated score [ 16] using 6 empirically weighted physiological measurements made during the first 12 hours after admission to the NICU.
