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We considered that the ratio of EGF loaded/unloaded EGFR in the vesicles is equal to the weighted ratio p-EGFR/EGFR on plasma membrane with weight factor w. Importantly, the use of this term in the model gave the best description of the time course of total EGFR, but was not essential to explain the p-EGFR dynamics in individual endosomes (data not shown).
The weighted ratio given by Equation (4) was preferable to estimate the speedup.
The weighted ratio of the minimum fitting GPS residuals was chosen as the optimal relative weight ratio in this interval.
Thus, the distribution of the speedups, calculated by the weighted ratio formula (4), had an approximation to a normal distribution.
and the weighted ratio R ¯ W is given by R ¯ W = X ¯ Y ¯ = ∑ X i / n ∑ Y i / n = ∑ X i ∑ Y i. (4).
This definition of speedup is the one adopted in this work, and it coincides to the weighted ratio definition in Equation (4).
Similar(46)
The relative enrichment of proteins in the peroxisomal fractions compared to total cell homogenates was quantified as average weighted ratios of the peak areas of respective peptides.
Binding ratios for each of the 2 replicated samples were combined by a weighted average method, and final p-values were calculated for the weighted ratios at the 0- and 3-hr time points [ 69].
Because the assets in this ratio are risk-weighted, it must be converted into a simple (un-weighted) ratio to make it historically comparable.
In addition, FC (ratios in log 10 scale) were also computed using an error-weighted ratio combination method.
Statistical significance of weighted rate ratio's are calculated using a Poisson regression model including an overdispersion parameter to account for variability in exacerbation rates between patients.
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