For measurement of muscle weights, individual muscles were dissected from both sides of the animal, and the average weight was calculated.
For dry-weight measurements, mouse brains were dissected intact, washed in PBS, blotted dry on Kimwipes, weighed wet, then incubated overnight in acetone.
Selected samples were dissected and weight of tissue samples recorded and kept frozen at −20 °C until experimental assays.
After thawing the lymph nodes, calculation of wet weight was performed, and specimens were dissected and submerged in thioglycolate broth (Oxoid, Wesel, Germany).
After the mice were sacrificed at 12 days after the collagen booster the different skeletal muscles form the hind legs were dissected and weight.
From the second part, tumor and contralateral brain tissue fragments were dissected (average weight 93 mg) and transferred into cryotubes and frozen in liquid N2.
The heads from adult cadavers of 4 green iguanas (3 females and 1 male, mean length 85.1 ±18.1 cm, mean weight 2824 ± 856 g), 4 tegus (2 females and 2 males, mean length 42.5 ± 15.1 cm, mean weight 1587 ± 785 g) and 4 bearded dragons (1 female and 3 males, mean length 18.1 ± 7.1 cm, mean weight 385 ± 49 g) were dissected for this study.
For measurement of muscle weights, individual muscles from both sides of the animal were dissected, and the average weight was used for each muscle.
A 0.1µg dose in 10µl acetone of the JH-analog methoprene was topically applied on three successive days to feeding larvae, starting at weights between 9 10g, and disks were dissected 24h after the last treatment.
The xenografts were dissected and weights were measured 24 days after inoculation.
