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Briefly, leaves (approximately 0.02 g fresh weight) were cut and marinated in 5 ml of 5 4 1 acetone: ethanol: H2O for 18 h under dark conditions.
The bands with appropriate molecular weight were cut out and digested by trypsin according to Havlasova and colleagues [ 27].
In brief, 20 to 60 cryostat sections of 30 µm size corresponding to 30 to 100 mg weight were cut from frozen tissues.
Detached leaves (0.5 g fresh weight) were cut and weighed immediately to obtain fresh weight (FW), and were then placed in a beaker filled with water overnight in the dark to obtain turgid fresh weight (TW) the next morning.
Leaves (approximately 0.02 g fresh weight) were cut and soaked in 5 mL of acetone (5): ethanol (4): H2O (1) miscible liquids for 18 hr in the dark, then residual plant debris was removed by centrifugation.
Snap-frozen blocks of brain tissue from MS and control cases of between 0.5 g and 1 g wet weight, were cut and re-suspended at 1 5 g/mL in Tris HCl buffer (100 mM Tris, pH 8.1 with 1% Triton X-100).
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Although the hardtop was already the stiffest, lightest and fastest Corvette, Chevy reached into its bag of tricks to create the Z06: The 5.7-liter V-8 engine was reworked to produce 385 horsepower, the suspension was revised and the unloaded weight was cut to 3,115 pounds -- almost 100 pounds lighter than the '01 coupe and convertible and 38 pounds lighter than the previous hardtop.
The nitrocellose bands corresponding to the molecular weights were cut and counted in a Beckman gamma counter.
VF and DR are highly sensitive tests for distinguishing MCI from NC [ 14], and although their weighting were cut, by a factor of 0.66 and 0.8 respectively, to allow for the introduction of LM, their relative weighting, compared to the other subtests, increased.
France Télécom's weighting was cut to 30percentt, Deutsche Telekom to 40percentt and Telecom Italia to 45percentt of their market share values.
For disintegration and solid release trials, rectangular prismatic sections (length × width × height = 6 × 6 × 4 mm, weight 0.15 g) were cut from raw and roasted almonds.
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CEO of Professional Science Editing for Scientists @ prosciediting.com