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Compost samples were prepared for total coliform and E. coli population estimates as follows: each sample was thoroughly mixed and a subsample of 10 g (dry weight) was weighed into 90 ml of sterile phosphate-buffered saline and shaken vigorously for about 2 min.
And body weight was weighed every 7 days.
Rossete leaves of identical size and stage were severed and fresh weight was weighed before and after exposed in air.
Four weeks later mice were sacrificed, tumor was resected and then their weight was weighed respectively with electro-balance.
Frozen fruit powder (1 g fresh weight) was weighed in three replicates into a 20-mL crimp cap vial; the vial was closed and incubated at 30 °C for 10 min.
Stool weight was weighed three times per sample using an electric balance, whereas the water content was determined as the difference between the wet and dry weights of the stool as described previously [ 6, 11].
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Besides, right tibia lengths were measured using a digital caliper and the left heart weight were weighed.
Tissue samples (∼2 10 mg of wet weight) were weighed, dried, and homogenized in 1 mL of water using a 24-bead homogenizer.
The homogenized wheat ears (100 ± 2 mg fresh weight) were weighed into Eppendorf tubes and extracted with 1 mL of precooled methanol/water (75 + 25 (v/ v)) including 0.1 % formic acid by vortexing for 10 s and further treatment in an ultrasonic bath at room temperature for 15 min (resulting in a dilution of 1 10 (w/ v)).
Dry weight (DW) was weighed after drying the leaves to a constant weight in an oven at 80 oC.
To determine if the anorectic effect of visfatin affects body weight, rats was weighed immediately prior to visfatin injection and 24 h after the injection.
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