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The next day transfer of high molecular weight samples was ensured at 450 mA for one hour.
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For this, different molecular weight samples were pre-sheared and then crystallized at the same temperature for a specific period of time.
The capability of this instrument to obtain information about the molecular weight distributions of heterogeneous high molecular weight samples is demonstrated with 8 MDa polyethylene glycol (PEG) and 50 and 100 nm amine modified polystyrene nanoparticle samples.
To estimate the molecular weight, samples were run alongside a biotinylated protein ladder (Cell Signaling Technology).
Dust weight samples were available for 829 households, and survey information was available for 2,456 participants (children and adults).
For dry weight, samples were collected in 2 mL pre-dried, pre-weighed micro-centrifuge tubes, washed twice with equal volume distilled water and dried at 100°C.
For dry weight, samples were collected in 2-ml pre-dried, pre-weighed microcentrifuge tubes, washed twice with equal volume distilled water and dried at 100 °C.
One equal weight sample was kept in the dark throughout the whole experiment.
A known weight sample was incubated in 1.6 μg/mL of lysozyme solution (pH 7.4) at 37°C.
In order to create a reference dataset for NC, only the healthy-weight sample was considered.
The weight of samples was measured by the digital balance (Sartorius balance, BP 210 S, German).
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