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Each gel lane was cut into 4 equal slices using a scalpel, and labeled as A, B, C, and D from high to low molecular weight regions.
Images were cropped to show detected bands at correct, expected molecular weight regions, and then converted to grayscale and subjected to automated contrast adjustment using the default program settings.
Each sample was fractionated into nine molecular weight regions.
After destaining, gel lanes were cut into three molecular weight regions.
The nitrocellulose was cut into molecular weight regions (blots) and then solubilized to form nitrocellulose particles.
The CBB stained bands matching the expected molecular weight regions of selected proteins were excised and subjected to Trypsin digestion.
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As shown in Figure 4, as time increased, the GPC curves shifted from the lower molecular weight region to the higher molecular weight region due to the CRP mechanism.
According to GPC-IR, 13C NMR and CEF characterization results of some polymers, more 1-hexene was incorporated in the high molecular weight region, short chain branches were generated by the chain walking mechanism in low molecular weight region.
The gel permeation chromatography (GPC) measurement of the polyrotaxane in DMSO exhibited peaks in the high molecular weight region.
The molecular weight distribution curves gradually shifted to the low molecular weight region with a narrower distribution and the chain degradation reaction followed the random scission model.
The TG curve reveals the occurrence of a marked weight gain in the 375.18 997.84 °C range, following by a weight loss from 997.84 to 1040.13 °C and then slowly decreases weight region of 1040 1200 °C.
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