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The degree of compression, and thus the weight of solution dispensed, is controlled by a microprocessor-controlled motor-drive mechanism.

Hydrolysis simulations were performed based on the experimental conditions: weight of solution (scale of hydrolysis), solid loading, cellulose content, total enzyme loading (mg protein/g cellulose), ratio of enzymes present (EG CBH I CBH II:BG), temperature, pH and hydrolysis duration.

3. Euthanize animal with CO2 or inject mouse IP with 0.01 mL/g body weight of solution (B).

Hydrolysis simulation was initialized based on the weight of solution (scale of hydrolysis), solid loading, cellulose content, total enzyme loading (mg protein/g cellulose), ratio of enzymes present (out of eight classes), temperature, pH and simulation time (hydrolysis duration).

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Briefly, mice were treated with 0.01 ml/g body weight of solutions containing either only vehicle [4 % carboxymethylcellulose (Sigma-Aldrich, Germany) and 1.25 % chloroform (Carl Roth, Germany)] or rotenone (0.625 mg/ml rotenone (Sigma-Aldrich, Germany), 4 % carboxymethylcellulose and 1.25 % chloroform) once a day, 6 days/week for 4 months.

However, most present phosphorylation strategies for chitosan modification are accompanied by some decrease in molecular weight of chitosan and hence the effect of molecular weight on solution properties could not be completely eliminated.

Once the weight of the solution is balanced by the surface tension, the solution will stop flowing from the top and cause saturation at the end of the flowing, which causes a convex meniscus (Figure 2c).

The optimization problem associated with an and/or graph G consists of finding a minimum weight solution subgraph H of G, where the weight of a solution subgraph is the sum of the weights of its edges.

The weight of a solution is used for determining the attractiveness of a solution to be chosen in the next evolution and is given by Eq. (22) (Schlueter 2012) w_{j} = frac{1}{{qksqrt {2pi } }}exp left( {frac{{ - ({text{rank}}(j) - 1)^{2} }}{{2q^{2} k^{2} }}} right) (22)In the equation, the term ({text{rank}}(j)) is given the rank of the solution.

Working standards were prepared by weighing 0.76, 1.53, 3.10, 5.89, and 11.34 g, respectively, added aliquots of the standard solution into separate 50 mL volumetric flask and diluting to 25 g final weight of the solution with distilled water to produce concentrations of 0.594, 1.19, 2.40, 4.58, and 8.80 mg/L, respectively.

All the aliquots were diluted to 10 g of final weight of the solution with distilled water to produce concentrations of 1000, 2000, 3000, 4000, 5000 and 6000 mg/L standard solution, respectively, for the FT-IR-ATR calibration measurement.

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