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The methyl ester yield was determined using GLC and % yield method as follows: (1) % yield = weight of biodiesel formed (g ) Total weight of reaction mixture (g ) × 100.
Finally, the linear objective is formed by multiplying the fluxes by an objective vector f, whose jth element f j is the weight of reaction j in the biological objective.
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From the weights of the possible reaction, at step 2, in the previous algorithm, we calculated the probability of a reaction being selected as the weight of the reaction divided by the sum of the weights of all possible reactions.
Notice that the weights of reactions do not take into account known metabolic pathways, although this approach could be explored in future work.
The transesterification of WCO produced 57.92% maximum FAME yield at the optimum methanol to oil molar ratio of 6 1, catalyst weight of 2.5%, reaction time of 240 min and a reaction temperature of 64 °C.
The enzymatic reactions were performed in 2-mL microcentrifuge tubes, and the total weight of the reaction mixture in each tube was 1000 mg.
This reaction is catalyzed in the model by an unknown element "X" and the weight of its reaction is set to 1000.
The context weight of a reaction node is the sum of the transformed connectivities of the compounds present in the reaction but not represented by the node.
The aspen pretreatment in the microwave system was performed at 165 °C for 10 min with an addition of H2SO4 that resulted in a final concentration of 1%% (w/w) of the total weight of the reaction mixture.
A sodium citrate buffer (50 mM citrate, pH 5.2) was added so that the final weight of the reaction mixture was 1000 mg.
The total weight of the reaction mixture was 1,670 mg and it contained 50 mg of the cellulosic/hemicellulosic sample, 50 mg enzyme mixture (a 1 1 ratio of a mixture of Celluclast 1.5 L and Novozyme 188, both of which were obtained from Sigma-Aldrich) and 50 mM citrate buffer (pH 5.2).
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