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However, the molecular weight of mouse SIRT3 is controversial.
The treatment group (N = 4) was changed to a diet containing 50 mg capsaicin/kg food (which is about 10 mg capsaicin/kg body weight of mouse per day).
At day 35 (14 day post the last immunization) mice were anesthetized by i.p. injection of ketamine and xylazine (at 0.1 mg and 0.05 mg/g body weight of mouse, respectively, in 0.25 ml injectable saline) and intranasally challenged with 3.4×104 colony forming units (CFU) of F. tularensis LVS in 50 µl saline.
On PND 1, the sex and weight of mouse pups were determined.
The ratio for the injection (amount of compounds/the weight of mouse) was 2 mg per 1 kg.
The weight of mouse pups was analyzed by descriptive statistics and t test (Statistica software, StatSoft, Inc., Tulsa, OK, USA).
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The weight of mice was measured and the condition of mice was observed every day.
We calculated the average body weight of mice for the experiments.
Additionally, combination strategy succeeded in maintaining the body weight of mice (Fig. 4D), suggesting an improved toxicity of this strategy.
In ethaselen-loaded polymeric micelle treated groups at all doses, the body weight of mice did not significantly increase.
a. Record weight of mice.
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