Mouse health records (weight at time of injection, duration(s) of injection, health over course of experiment, etc).
Data to be collected: Mouse records (weight at time of injection, duration(s) of injection, health over course of experiment, etc).
Tests require one-week observations for general health and weight following injection of specified volumes of product batches into guinea pigs and mice.
In addition to analgesic therapy, the main group of animals received a 5 μg/kg body weight subcutaneous injection of PGE1 (Vasaprostan) per day during 10 postoperative days at the contralateral thigh.
An analgesic (5 mg/kg body weight per subcutaneous injection of Rimadyl; Pfizer) and an antibiotic (5 mg/kg body weight intraperitoneal injection of Baytril; Bayer) were administered once per day for 3 days.
Animal weight is expressed as mean ± SD percentage of weight at injection.
For contrast enhancement, a 0.2 mmol/kg body weight bolus injection of gadopentate dimeglumine was administered.
After 4 days, the animals were sacrificed with a 0.1 mL/kg body weight intravenous injection of an embutramide-mebenzoniumjodide-tetracaine HCl solution (T61, Intervet, Belgium).
Male Wistar rats (232 ± 17 g) were anaesthetized by intraperitoneal injection of 20 mg/kg body weight sodium pentobarbital (Nembutal; Sanofi, Düsseldorf, Germany) and 30 mg/kg body weight intramuscular injection of Ketamin.
After surgery, animals received an analgesic (2.5 mg per subcutaneous injection of Rimadyl; Pfizer, New York, NY, USA) once per day for 5 days and antibiotics (1.25 mg/250 g body weight intraperitoneal injection of Baytril; Bayer) once per day for 3 days.
