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For all traits except percent weight gain, maternal effects were strong, and an important part of them was related to the initial weight of eggs.
Although she uses measuring cups rather than balancing the weight of eggs, flour and sugar, as the book calls for, her sponge cake, made with prepared matzo cake meal, is pretty much the same.
Differences in anti-Bm86 GMT, in the weight of engorging females, and in normalized weight of eggs laid in each immunized group were determined from weeks 0 to 27 using the non-parametric Friedman's test.
In addition, no statistically significant differences were detected in the average weight of eggs laid by ticks engorged on immunized cattle that showed anti-Bm86 specific titers in the range of 1 640 to 1 81920.
The analysis of the normalized weight of eggs laid also showed lower mean values at the end of the assay with the three implemented schemes (Mann-Whitney's test, p < 0.05).
For the embryo library, total RNA was extracted from approximately 1 g (wet weight) of eggs from each collection using Trizol (Invitrogen) according to the manufacturer's instructions, followed by treatment with DNase (DNAfree, Ambion).
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The following parameters were assessed weekly: total egg production, hatchable eggs, egg specific gravity, weight of egg shell, yolk, and albumen, as well as eggshell thickness.
The bags were dissected to determine the mating status of females and the weight of egg masses.
The weight of egg masses laid by mated females was determined for different sites and sampling intervals.
However, reproductive phenotype, shown as blood feeding periods, engorged body weight, pre-oviposition period, oviposition period, weight of egg mass per tick and egg-hatching rate were significantly affected by LKR- and SDH- dsRNA treatments (Table 1).
The phenotypic differences between the dsRNA-injected and control groups was investigated by measuring the attachment rate, the blood feeding periods, total number of ticks engorged, engorged body weight, mortality, pre-oviposition period, feces excretion, oviposition, weight of egg mass per tick and percent of eggs hatching.
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