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Analyte results are then reported as weight of analyte per gram of creatinine (micrograms analyte per gram creatinine).
Concentrations of pesticides or their metabolites in biological samples are usually reported as the weight of analyte per volume sampled (e.g., milligrams per liter).
The theoretical Rmax is based on the formula Rmax = (MWA/MWL) x RL x Sm where MWA/MWL is the ratio of molecular weight of analyte to ligand, RL is the amount of ligand bound in RU, and Sm represents the proposed stoichiometry of the interaction.
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Consequently, the m/z of the peak corresponds to the molecular weight of the analyte.
The recorded responses were double referenced (right channel, buffer blank) and normalized using the molecular weight of the analyte (in kDa).
The second esterification also increases the molecular weight of the analyte by 98 amu, which increases the selectivity in the MS detection since most interfering compounds from the urine matrix have lower molecular weights.
The LS detector is used to determine absolute molecular weight of the analytes from the ratio of the light scattering signal to the IR concentration signal.
Since the detection of molecular interactions using this technology is highly dependent on the molecular weight of the analytes [ 42], rather that attempting the direct measurement of the interaction of CBEL with various low molecular weight single sugars, we decided to check whether single sugars could interfere with the binding of large glycoproteins to CBEL.
Mean absolute recoveries ranged from 32±7%7% to 68 ± 18%, with recovery generally increasing with molecular weight of the target analyte (Table 1).
We estimated the population average intake rates of parent compounds from the AM analyte levels in urine, taking into account empirical data on fractional excretion of the analytes following ingestion exposure and also accounting for the relative molecular weights of the analytes and the parent compounds.
The exact weight was noted for calculation of analyte concentrations.
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