Male 8 – 10 weeks old NOD.B10 and NON mice were given intraperitoneal (IP) injections of bovine serum albumin (BSA, 1 g/100 g body weight) (CAT# A-7906; Sigma Chemical Company, St . Louis MO, USA) in saline (25% solution weight/volume) or normal saline 5 days a week, for 10 weeks and sacrificed 10 weeks post treatment.
To achieve weight loss, cats were fed 60% of their estimated maintenance energy requirements, calculated according to the NRC equation for obese cats [ 20], which equaled 326 MJ (body weight, in kg 0.4.
This is similar to the situation with dogs, but the relatively smaller size and weight of cats frequently results in symptoms that are less pronounced.
The choice of BN52021's concentrations was based on the clinical experience and the body weight of cats [ 14].
The age and weight of cats were compared with the calculation of means and Student's t-tests.
Mean weight for cats in Drourrs study was 5,5 ± 1,33 kg compared to 4,9 ± 1,3 kg in our study.
For histology, mice were anesthetized with an intraperitoneal injection of 2.5% Avertin (400 600 mg/kg body weight; Sigma, Cat. No. T48402) and perfused intracardially with 0.1 M phosphate buffer saline (PBS; Sigma, Cat. No. P3813) to remove the blood and then with 4% paraformaldehyde (PFA; Sigma, Cat. No. 441244) in 0.1 M PBS.
Did he spend five years tying tiny lead weights to cats, then dropping them off of rooftops, then slicing them open to test the impact velocity?
To investigate the influence of cholinergic signaling on behavioral arrests, 20 mice (n = 10 DKO; n = 10 WT) received IP injections of either physiological saline (0.9% NaCl) or physostigmine dissolved in saline (0.01, 0.03, and 0.08 mg/kg body weight; Fisher Scientific cat # 50809809) a half hour prior to video recording.
