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The monoisotopic masses of the neutral losses were recalculated using the Molecular Weight Calculator software.
The molecular masses of TaSUT2A, TaSUT2B and TaSUT2D were determined by using protein molecular weight calculator software (http://www.sciencegateway.org/tools/proteinmw.htm).htm
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Accurate mass calculations were performed using a molecular weight calculator, which is free, downloadable software from the Pacific Northwest National Laboratory [ http://omics.pnl.gov/software/MWCalculator.php].
A total of 10 descriptors were calculated using PowerMV [28] descriptor calculator software namely molecular weight, XLogP, PSA, H-bond Donor, H-bond Acceptor, number of Rotatable Bond Count, number of rings, number of nitrogen atoms, number of oxygen atoms and number of sulphur atoms.
Ideally, use a graphing calculator or calculator software to calculate the entire formula in one line.
Hardy-Weinberg equilibrium was calculated using the Hardy-Weinberg Calculator software as implemented in http://www.tufts.edu.edu
The determined values were compared with the conductivity values calculated by a pH & Conductivity Calculator software.
This information was used to calculate KA/KS ratios for positive selection using kaks calculator software [ 135].
Power calculations were determined using power and sample size calculator software PS version 2.1.31 http://www.mc.vanderbilt.edu/prevmed/ps/.edu/prevmed/ps/
(if you want to calculate the chargeable weight of your shipment, then check the chargeable weight calculator at www.freightfly.com).com
Molecular weights for the native conifer proteins and for the recombinant hybrid proteins were estimated by a peptide mass calculator software (www.peptidesynthetics.co.uk).co.uk
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