The device is based on three piezoelectric weighing cells located in a triangle with a sensitivity of ±1 N and a maximum load of 4 kN per cell.
Measuring the density of living cells is tricky because it requires a tool that can weigh cells in their native fluid environment, to keep them alive, and a method to measure each cell in two different fluids.
Wet cell-weight of the washed yeast cells was determined by weighing the cell pellet.
The wet-cell weight was then determined by harvesting the washed cells by centrifugation at 3,000 × g for 5 min and weighing the cell pellet (the estimated dry-cell weight for all strains was approximately 0.15-times the wet-cell weight).
The wet-cell weight was then determined by harvesting the washed cells by centrifugation at 3,000 × g for 5 minutes and weighing the cell pellet (the estimated dry-cell weight for all strains was approximately 0.15 times that of the wet-cell weight).
The wet cell weight was determined by weighing a cell pellet obtained from centrifugation of the pre-culture at 3 000 × g for 5 minutes.
The wet-cell weight was then determined by harvesting the washed cells by centrifugation at 3,000 × g for 5 min and weighing the cell pellet.
Dry cell weight (DCW) was determined by weighing dry cells from 10 mL of culture broth as the mean of triplicate measurements.
Cell concentration, defined as cell dry weight (CDW) per liter of culture broth in each time interval during cultivation, was determined by filtrating 10 mL of culture broth using tarred membranes filters (Millipore, 0.45 μm filters), washing cell pellets, drying at 80°C for 48 h, and weighing dry cells as previously reported (Pantazaki et al. 2009).
When the statistics was weighed by cell type content under various assumptions of transcription levels in adipocytes and stromal cells, we identified a set of re-occurring genes that were differentially expressed between the DM and NoDM group.
