Sentence examples for weeks of sphere from inspiring English sources

To this end, at the end of 5 weeks of sphere propagation (8 weeks from derivation) the hNPs were placed on laminin coated glass coverslips for a period of 12 days to allow terminal neuronal differentiation (Figure 1A).

After 2 weeks of co-culture, floating sphere cells were dissociated with 0.25% trypsin-EDTA, filtered with a 40-µm strainer to obtain the single cell suspension, resuspended in Matrigel (BD Biosciences, Bedford, MA) with KSFM (1 1, vol:vol), plated in the 24-well plate, and incubated at 37°C overnight to solidify before adding 1 mL KSFM.

After 8 15 weeks of differentiation the cell spheres were dissociated and replated in NDM on human laminin coated wells or MEA dishes.

The cells were incubated in a 5% CO2 incubator for 2 weeks and the number of spheres was counted under a microscope in 15 low-power fields and then the average was calculated.

After 1 or 3 weeks of incubation with GN-25, secondary spheres were harvested for counting as described above.

Quantification of sphere growth and passaging.

For determination of sphere size, we gauged sphere diameter optically with an object micrometer.

After 3 weeks of MEC co-culture, single SCs formed spheres with a highly branched ductal-like structure in Matrigel indicative of an aggressive phenotype.

The number of wells containing at least one secondary sphere was evaluated after 3-4 weeks of culture.

However, YES-2S cells were only able to form spheres after two weeks, and the average number of spheres was 4.3 ±5.13 (mean of all spheres in 3 plates ±SD) with diameters ranging from 40 100 μm.

After 3 weeks of co-culture, single SC were seeded into uncoated 6-well plates until free-floating spheres formed.