One mini-implant in the RVG group was removed within the first week of loading due to increased mobility and, hence, was considered a failure.
One mini-implant in the RVG group, which was removed within the first week of loading, was not in contact with any of the neighboring roots but was placed relatively closer to the arch wire.
We also investigated the effects of intermittent parathyroid hormone (iPTH) on the (re)modelling response to 2-weeks of loading and the effect of 2-consecutive days of loading on osteocytes' sclerostin expression.
By contrast, in the H IGF-I KO mice with partial estrogen loss, TV increased by 8.8%, BMD by 4% and cortical thickness by 6% in the loaded tibia when compared to non-loaded tibia after 2 weeks of mechanical loading.
Although inflammatory cytokines in the forelimb bones (radius and ulna, and first row of carpal bones) were effected mainly by force levels, with increases of several cytokines observed in both LRHF and HRHF rats, it was clear from the morphological studies that bones responded catabolically to the 12 weeks of HRHF loading, and anabolically to the LRHF and HRLF loading.
Two weeks of mechanical loading increased BV/TV, trabecular thickness and trabecular BMD in the loaded tibia by 12%23%% when compared to non-loaded tibia in all four groups of mice but there was no significant difference in the bone response between the groups in response to loading.
Two weeks of mechanical loading caused a 7%–8% and an 11%13%% (P<0.05 vs. non-loaded bones) increase in cortical BMD and cortical thickness (Ct.Th), respectively, in the control sham, control pOVX and H IGF-I KO sham groups.
Two weeks of axial loading caused similar increases in trabecular BV/TV (13%17%%) and thickness (17%–23%) in all four groups of mice.
Importantly, in vitro analysis demonstrates that H2B-GFP+ and rare unlabeled H2B-GFP− SCs isolated after 6 weeks of Dox loading are functionally indistinguishable (supplementary material Fig. S3B).
As shown in Figure 3, in response to 2 weeks of axial loading, BV/TV, trabecular thickness and trabecular BMD were increased by 5%23%% in all four groups of mice.
