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Each experiment lasted nine weeks (five weeks of adaptation and four weeks for data collection).
Average ruminal pH was lower (P=0.0001) during SARA than during the weeks of adaptation (5.6 versus 6.1).
Each period consisted of 2 weeks of adaptation followed by 1 week of measurement with the CO2R and CO2T.
After 2 weeks of adaptation period, pharyngeal portion was shaped using high-viscosity impression wax during production of oral pressure sounds which cause velopharyngeal function.
The periods consisted of 2 weeks of adaptation (day 1 14), week 3 of baseline measurements (day 15 21) and week 4 of acidosis challenge (day 22 28).
Each period of the Latin square consisted of three weeks of adaptation to the respective treatments followed by four days of induction of SARA and three days of rest.
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The objective of this study was to compare the short-term (first week of adaptation to a new ration) behaviour of dairy heifers when fed a low-concentrate, high-forage ration ad libitum to when fed a high-concentrate ration in a limited amount followed by ad libitum access to straw for the remainder of the day.
After one week of adaptation, two groups of animals (control, n = 24; infected, n = 24) were constituted.
Fifty-six old females and 16 males (aged 8-weeks) were caged by 2 and given commercial pellets (Safe 113) for one week of adaptation.
After 1 week of adaptation, the mice were inoculated s.c. in the scapular region with 2×106 CNE-2 cells or 1×107 C666-1 cells to generate tumors for the following experiments.
After one week of adaptation, the mice (n = 20) were randomly divided into four groups of five mice each.
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