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The inclusion criterion for both groups was that the last anthelmintic treatment had been administered at least 56 days (8 weeks) before sampling.
Animals were maintained seven weeks before sampling pituitary glands.
Only 4.5% (52/1145) of strategically treated horses were dewormed >36 weeks before sampling: 49 horses 37 48 weeks before sampling and three horses >48 weeks before sampling.
The brains of the autopsied subjects were fixed in phosphate-buffered 4% formaldehyde for at least 2 weeks before sampling.
Growth of COX + and COX++ lines was slightly retarded during the first 4 weeks after transplanting, but this difference disappeared in the last 2 weeks before sampling.
They also administered their questionnaire on sampling day 1, whereas the NHANES household pesticide use questionnaire was administered 1 3 weeks before sampling.
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Residents were instructed not to sweep or vacuum the week before sampling.
The fish were fed ad libitum with Crangon crangon once a week ; feeding was terminated exactly one week before sampling.
To kill potential tapeworm eggs, the carcasses were placed in –80° C for at least one week before sampling.
From two of the seven animals of the late leukemic stage, the blast count could not be determined because the two mice died at the age of 22 to 24.5 weeks shortly before sampling.
Intra-articular injection was discontinued for at least 2 weeks before sample collection.
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