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Ten of the remaining 91 reported persisting symptoms at the 6-month follow-up; three of those were excluded due to presence of symptoms >3 weeks before inclusion, resulting in a total of 88 patients (55 women, 33 men, median age 57 years), seven (8%) of whom reported persisting symptoms 6 months post-treatment.
Antiandrogen therapy was discontinued >4 weeks before inclusion (>6 weeks for bicalutamide).
In 95 patients clinical symptoms started earlier than 6 weeks before inclusion into the study.
The 21 women in our study did no use antibiotics for at least 6 weeks before inclusion in the study.
In the remaining 24 patients, the onset of clinical symptoms was within 6 weeks before inclusion and these patients were classified as suffering from "acute" disease.
Radiotherapy should have been completed at least 4 weeks before inclusion, and patients should have recovered from any toxic side effect.
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The number of drugs (http://www.bcfi.be) taken during the week before inclusion was recorded to detect polypharmacy (≥5 different drugs).
Patients were required to have pain of 55 mm or greater on a visual analogue scale (VAS) at any time during the week before inclusion.
Patients were excluded if they had been treated with antibiotics up to 1 week before inclusion or if they had a history of hospitalization within the last month.
The overall daily caloric intake in the week before inclusion into the study was 1693 ± 841 kcal and during the study period 2211 ± 543 kcal (P = 0.002).
The animals were acclimatized in a separate room, under controlled lighting and heating conditions, in Experimental Animal Resources Center for 1 week before inclusion in the study.
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