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For ST30 cells, 30nm paclitaxel was withdrawn at 2 weeks before experiment.
The cells, 5 × 10 in Matrigel, were implanted in the right hind leg 2 weeks before experiment.
Pigs were transported to the animal facilities of the Polish National Veterinary Research Institute two weeks before experiment (acclimatisation period).
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Planarians were starved for 1 2 weeks before experiments.
Uninfected normal red blood cells (NRBC) used as controls were cultured the same way for at least 2 weeks before experiments.
Rats were randomly divided into eight groups of five animals and acclimated 2 weeks before experiments.
Animals were handled 2 weeks before experiments and every 2 to 3 days throughout the study.
The cells were cultured without 5-fluorouracil for two weeks before experiments.
Three- and 7-week-old C57BL6 mice were purchased from Taconic Farms, Inc. (Hudson, NY) and acclimatized for 1 2 weeks before experiments.
The mice were 12 weeks old and acclimated in a quarantine room for 2 weeks before experiments, and maintained on a standard pellet diet.
Athymic female nude mice (BALB/c nu/nu, aged 6 8 weeks) were purchased and allowed to acclimatize for 1 2 weeks before experiments were started.
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CEO of Professional Science Editing for Scientists @ prosciediting.com