Exact(2)
Wells with single cells (118 total) were marked and incubated in medium with serum with weekly medium changes for a month.
The number of colonies increased with incubation time in both methods and the weekly medium replenishment in the Courtenay method was advantageous for longer incubation times of several weeks.
Similar(58)
The first fluorescent colonies of bacteria were noted 6 wk following electroporation, and the culture was maintained in selection medium with twice-weekly medium changes until ~90% of cells were infected.
The wt-MCF7 cells were passaged weekly and medium was replenished every two to three days.
For mechanistic studies LTED cells were passaged weekly and medium was replenished every two to three days.
Ap-infected ISE6 cultures were fed twice weekly with medium buffered to pH 7.6 using 0.25% NaHCO3 and 25 mM HEPES, and subcultured 1 50 bi-weekly [ 13].
Cells were cultured for 6 weeks with weekly half-medium changes then washed, counted and plated for colony scoring as described above.
Visually confirmed single cells were added onto irradiated UG26 feeder cells in flat-bottomed wells containing 200 μl of MyeloCult (STEMCELL Technologies) supplemented with 10−6 M hydrocortisone (Sigma-Aldrich) in 96-well plates and cultures maintained with weekly half-medium changes (Woehrer et al., 2013).
Because of the nature of this dataset, better overall model performance could be achieved when different training and testing sets were used for training a classifier for medium weekly case numbers than those testing sets used for training classifiers for high weekly case numbers.
Soft agar cultures were incubated at 37°C/5% CO2 for 3 weeks and fed weekly with fresh medium.
Cells were passaged weekly, with a medium change once between passages.
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