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Ten weekly experiments were conducted.
A total of 40 animals were challenged with 24 h ET infusions in ten weekly experiments (four animals per experiment).
Twelve animals were challenged with 24 h infusions of LT and ET together in three weekly experiments (four animals per experiment).
The animals were randomly assigned to receive intravenous immune globulin (IVIG) as control or anthrax immune globulin (AIG) at T-4 or T2 in six weekly experiments, at T2 or T5 in one experiment, and at T5 in one experiment.
Experimental participants rated each of the Step Up program components as at least moderately helpful, although aspects of the program that involved the health coach and the calls were rated more highly than the program workbook or weekly experiments (Table 2).
Participants did not have to be ready to take action on any of the three target behaviors in order to be eligible for the study, just willing to talk about these issues each week with the counselor and agreeable in principle to trying the weekly experiments.
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We analysed experimental population growth in both experiments 1 and 2 by fitting Linear Mixed Models with a Gaussian error distribution (command MIXED) in SPSS 15.0, using the monthly (experiment 1) or the 3-weekly (experiment 2) population count of each tank as a repeated measure.
These cells were maintained in Dulbecco's modified Eagle's medium (Thermo Scientific) with 10% foetal calf serum (Trace Scientific) and 500 µg/ml Geneticin (Invitrogen) at 37°C and passaged weekly.All experiments were conducted in growth media with 10% foetal calf serum.
Cells were passaged weekly and experiments were performed within 1 2 passages.
The osteogenic medium was changed weekly and experiments were stopped after 7, 14 or 21 days to assess the osteoblastic phenotype of MSC.
Hence, in Experiment 1A all tanks were replaced with clean tanks weekly and in Experiment 1B twice throughout the experiment when siphoning did not lead to satisfactory results.
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