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After 1 week, PM was isolated from a bronchoalveolar lavage fluid sample, and the tracheal protrusions were cultured.
At 1 week, PM secreted growth factor(s) for MC, and the mitogenic effect was more pronounced with lavage fluids.
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Female mice were caged for 1 night with EcoHIV/NDK-infected males and, after 1 week, PMs were collected to measure virus burden: HIV gag RNA was detected in all females (Fig. 2C).
These data indicate that male and female X. tropicalis reach reproductive maturation at 22 and 30 weeks PM, respectively.
Vitellogenic oocytes increased in number up to 15,000 per female at 30 weeks PM and accounted for 75% of the total number of oocytes present in the ovary.
The ovary and oviducts continued to grow in mass until 30 weeks PM at which point they had reached sizes of 3.6 g and 0.8 g, respectively.
Testes sizes in males reached their peak at 22 weeks PM (21 mg) with sperm counts peaking at the same time (25 million sperm/male).
Male frogs exhibited nuptial pads, starting at 8 weeks (PM) when measureable concentrations of circulating testosterone (5.3 ng/mL plasma) first appeared.
In females, estradiol becomes elevated in the blood at 16 weeks PM (1.5 ng/mL plasma) which corresponds with the presences of vitellogenin (4.4 mg/mL plasma), vitellogenic oocytes in the ovary, ovarian growth, and oviduct growth.
In these hyperlipidemic rabbits, 4-week PM exposure was associated with the progression of atherosclerotic lesions, coupled with an enhanced release of bone marrow monocytes.
Between February 2009 and February 2010, three 2-week PM measurements were conducted at each site in the spring/fall, summer, and winter months, resulting in a total of 120 samples.
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