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After a minimum of 1 week of acclimatization, the mice (n = 5/group) were randomly divided into four groups.
After a minimum of 1 week of acclimatization, the mice (n = 4/group) were randomly divided into four groups.
After 1 week of acclimatization to the chambers and the experimental diet, feces were collected for the next 45 days to ensure that there were sufficient samples to perform all the evaluations.
Adult male Wistar rats (250 270 g; SLC Inc., Shizuoka, Japan) were housed individually, cared for in an animal facility and fed a Conventional diet (Oriental Yeast Co., Ltd ,Ibaraki, Japan) with free access to water from the first week of acclimatization to habituation to treadmill running.
Following 1 week of acclimatization to the housing environment, rats were exposed to their respective exercise programs (see Table 1 for details of the exercise programs) on a Columbus Instruments Exer-3R treadmill (Columbus, OH, USA) for 12 weeks.
After 1 week of acclimatization, weight-matched rats were divided randomly into four groups (n = 6 per group) comprising a sodium chloride group (control) and low-, medium-, and high-concentration groups (7.5, 15, and 22.5 mg/kg body weight and named SWCNTs-L, M, and H, respectively).
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After 2-weeks of acclimatization, six animals were sacrificed to obtain baseline parameters.
After 1-week of acclimatization, the animals were randomized into 3 groups.
Climbers face weeks of acclimatization time climbing slowly from camp to camp, thin air, treacherous terrain and freezing conditions.
After 3 weeks of acclimatization, the experimental rabbits were then randomized into five groups (A to E) of six (6) rabbits each.
Although Marion's eyes should be dilated after her death, the contact lenses necessary for this effect would have required six weeks of acclimatization to wear them, so Hitchcock decided to forgo them.
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