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After 1 week of acclimatisation, mice were divided into two groups: a control group (CT) fed with a control diet (low fat, no sucrose, matched, purified ingredient diet corresponding to D12450K, Ssniff, Germany; n = 9) and a group fed with a WD (high-fat diet corresponding to D12451, Ssniff, Germany; n = 18).
After a week of acclimatisation, mice were injected subcutaneously in the left flank with 1 × 106 A375 human amelanotic melanoma cells, in 100 μL of Ca2+/Mg2+-free phosphate buffered saline.
The animals were housed with a week of acclimatisation before procedures were done under pathogen-free conditions in a temperature- and humidity-controlled environment and given access to food and water ad libitum.
Experiments began after 1 week of acclimatisation.
Experiments were performed after 1 week of acclimatisation.
The mice at 6 weeks of age were used after 1 week of acclimatisation.
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After two weeks of acclimatisation in and around Everest base camp, he and his team of 12 were due to climb towards the summit that night.
After a couple of weeks of acclimatisation, a fit person with little technical knowledge can feasibly summit Mt Chimborazo, the highest in the country.
After 3 6 weeks of acclimatisation and after pregnant females had given birth and weaned their young, animals were behaviourally tested.
At least 2 weeks of acclimatisation at the altitude of competition is generally recommended before playing matches, but information on team sports is lacking.
What this study adds Two weeks of acclimatisation at high-altitude is not long enough for native sea-level players to fully recover their high-intensity running performance, despite resting measures of HR, HR variability and wellness having returned to normal.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com