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By using a steel weaving frame, the disadvantages of supercontraction could be avoided, which were considered as obstacle for biomedical applications [27].
The art depended upon a weaving frame, which allowed the manufacture of long strips, useful for necklaces, belts, panels, and headbands.
The maximum number of cells seemed to depend mainly on the individual specimen and thus the amount of spider silk reeled onto the weaving frame.
Therefore, our study has a pilot role describing the use of native spider silk fibres as a biomaterial, in particular as scaffold weaved on a weaving frame, potentially rivalling other biomaterials currently in use.
Here, cells grew densely on the central parts, in which spider silk fibres were aligned in a crosslink-pattern, while fewer cells appeared on the linear fibres on the peripheral areas of the weaving frame.
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Weaving frames were placed on the bottom of the Pluronic-coated culture dishes and seeded with 5×105 cells/ml for SEM and 5×103 cells/ml for other investigations.
To determine if changes in adhesion and proliferation occurred between days 1 until 5, weaving frames with spider silk were treated with trypsin to digest surface amino acid residues.
On day 5, however, spider silk weaving frames were densely seeded with cells.
The variances between the specimens were very high, particularly on the weaving frames (Table 1).
The weaving frames manufactured in this study together with the network of spider silk woven on them were suitable to achieve a two-dimensional scaffold for the purpose of investigating interactions between spider silk and cells.
As well as creating arty selfies, users of the Prisma iPhone app can now turn snippets of video up to 15 second long into playable animations by selecting one of the nine filter styles currently available — and waiting for the app's AI algorithms to weave a frame by frame transformation.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com