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We examined whether the inability to induce natural peripheral tolerance to an allogeneic tissue was absolute or if instead tolerance might be established to weakly mismatched internal transplants; the exposure of skin grafts to the external environment, or other factors may reduce their ability to establish peripheral tolerance.
Together these data suggested that an internal weakly mismatched transplant is not ignored by a newly generating immune system, instead the location of the established graft determines whether the graft triggers CTL priming or tolerance.
In a separate set of B6-RAG-KO female recipients, not shown in the manuscript, we confirmed our earlier data [ 2] that the immune reconstitution with fetal liver cells is sufficient to restore the ability to reject even weakly mismatched (male) established skin grafts (skin grafts given pre-fetal liver).
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