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This method relies on the excitation of surface plasmons using a weakly focused beam with the average angle of incidence equal to the resonance angle, while the reflected light is detected using a bi-cell photodiode.
The second harmonic object field is generated through second harmonic scattering by a weakly focused fundamental laser beam from SH-active harmonophores in a specimen.
Weakly focused excitation light from a helium-neon laser filtered to select the 543 nm line is directed by a dichroic mirror onto the pinhole.
The output light was coupled into a multimode fiber (M30L02, Thorlabs), passed through a conical lens, and then weakly focused by an optical condenser into the sample.
In this approach AuNPs are deposited on cell membranes and irradiated by weakly focused femtosecond (fs) laser pulses, resulting in a significant increase of membrane permeability.
A Gaussian beam emitted from a single mode laser diode at a wavelength λL = 660 nm is linearly polarized and weakly focused at normal incidence on the sample by a microscope objective (10×, NA = 0.3).
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In addition, weakly focusing the excitation light to a spot larger than the pinhole further eases alignment tolerances.
A weakly spherical focused ultrasonic transducer can potentially be employed.
We then focused on weakly expressed sense loci (bottom third of all genes), for which potential antisense transcription would be more prominently reflected by Pol II.
Considering that expression values ranged from 0 to 15 in log2 scale and that 70% of miRNAs were weakly expressed, we then focused only on miRNAs with signal over 5, arbitrarily set to the average value of miRNA expression.
This study focused on the weakly nonlinear complementarity problems with a large sparse matrix.
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