XPS, SIMS, SEM and AFM analyses showed that under suitable conditions, He/BD plasma resulted in nano-scale chemical modification of the textile substrate instead of weakly adhered physical deposition of condensates of BD on the surface.
All methods mentioned so far are suitable for probing cells in an at least weakly adhered state.
Then, the supernatant of each well was removed and biofilms washed three times with 200 μL/well sterile water to remove cells weakly adhered.
Weakly adhered beads were detached under flow conditions, and some beads attached to the HUVEC layer were continuously concentrated to form EC clusters.
Stable-bleb cells only weakly adhered to and failed to migrate on 2D adhesive substrates coated with ECM components, such as Laminin and Fibronectin.
We discarded noisy force curves and/or curves that presented jumps possibly due to cantilever slippage or very weakly adhered and moving rounded cells.
White blood cells in the bloodstream weakly adhere to the endothelium lining the inner vessel wall (tethering) followed by rolling of leukocytes on the endothelial layer.
However, cardiomyocytes weakly adhere to these microposts, which limits the possibility of using biomechanical assays of single cardiomyocytes to study heart disease.
Our results support general matrix-dependent BMP signalling in terms of FAK activity since weakly adherent cells demonstrated poor BMP responses whilst well adhered and spread cells exhibited elevated BMP effects.
A drawback of this technology is that some anchorage-dependent cell types do not produce sufficient amounts of positively charged extracellular matrix proteins, adhering only weakly to the plastic substratum.
