PTD 6 was weakly active compared to the Tat and polyR motifs.
Alternatively, transcription of differentiation-specific genes might be attenuated, but still weakly active and rapidly switched to a more active state upon activation of respective signaling cascades, initiating and accompanying the transition into another differentiation state.
Nevertheless, data on viability, although important, are insufficient cells might be viable but weakly active and are unable to perform in fermentation.
That said, the interpretation of results from the analysis of microbial community composition using DNA-based methods can be confounded by the presence of DNA from dead, dormant, or weakly active organisms contributing little to overall microbial community function.
Therefore, the VMG proceeded with the design and execution of phase 2 to demonstrate the ability of the protocol to identify weakly active androgenic and antiandrogenic substances and 5α-reductase inhibitors.
As expected, we found Chlorpromazine (CPZ) and Quinacrine (QC), which are present in the Prestwick library, to be weakly active against yeast prions (Figure S1), as previously observed [16].
Since the 35S promoter seems to be only weakly active in arbuscule-containing cells [ 14], we used the MtPt4 promoter of M. truncatula[ 15], to enable a strong and specific expression of the amiRs.
Removal of the acetic acid moiety led to inactive or weakly active target compounds.
As previously reported, robotnikinin (1) proved to be only weakly active in this assay.
When one of the two methyl groups was eliminated from 4-isopropylphenol, the resulting 4-ethylphenol [HO-C6H4-CH2-CH3] was found to be very weakly active (289 nM) (Table 2).
