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Taken together, both immunogens induced cross-reactive antibodies to one or more VAR2CSA domains, but there was weaker recognition of the DBL4 domain in animals immunized with the DBL1-6 recombinant protein.
Also, in this study, recombinant antigen Asp f9 (mistakenly identified as Asp f16 in earlier studies) induced IFN-γ responses with weaker recognition by IL-4 or IL-17-secreting T-cells.
This would explain the weaker recognition of 85 A and 85 D. Furthermore, the comparison of the 3D structures of antigen 85 A and 85 B revealed that the glutamic acid in 85 B forms a pin structure in front of the "DPAW" region.
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Absence of the factors, such as universality, uniqueness, permanence, and acceptability, leads to a weak recognition system with high error rates.
Alternatively, the weak recognition of the EB preparation in the CTH1 vaccinated mice may relate to differences in the conformation of the two vaccine antigens in their natural localization in EB's compared to the recombinant vaccine construct.
This process implies a weak recognition of the polyadenylation DNA signal.
The antibodies to H3K4me1 and H3K4me3 were highly specific, while the antibody to H3K4me2 had weak recognition of H3K4me3 (Additional file 1, Figure S1).
Additionally recombinant Eng2 was highly recognized by sera pooled from aspergilloma patients while the negative control from healthy persons only showed weak recognition (Fig. 2).
Simultaneous analysis of multiple genomes from the same taxonomic group allows one to make reliable predictions of TFBSs even with weak recognition rules.
This study has clearly explained the weak recognition of H. pylori flagellin through TLR5 as an important immune evasion process of this long-term colonizing pathogen.
The weak signal obtained could result from a partial display of the recombinant protein or from a weak recognition by the first antibody because of unspecific interaction with the second antibody.
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