Cells from other normal organ (breast or ovarian) express a weak density of HER2/neu.
The head region of Pf40S has weaker density than the rest of the ribosome due to the inherent flexibility at the neck (centered around h28).
However, besides being at considerably lower resolution, "Domain 3," which includes PKI, had weaker density than the rest of the molecule.
Subsequent density modification by autoSHARP showed clear density for Spt5 and weak density for Spt4.
Difference maps designed to localize TEP1 showed only weak density inside of the caps, suggesting that TEP1 may interact with MVP via a small interaction region.
They also suggest that the weaker density may be a consequence of flexibility, which would allow it to move out of the A site.
Firstly, we were able to separate the weaker electron density of the detergent micelle from that of YidC.
4-fold NCS averaging was used to improve maps in the areas of weak density in chains C and D corresponding to the linker and the β3−α2 and β2′−β3′ loops.
Review 1: 1) Figure 2, and discussion of weak density in the scanning complex: It is unclear why it could be " possible that the nascent chain is present at lower occupancy" considering that the RNCs are purified by the 3x-FLAG tag on the nascent chain.
Specific comments: 1) Figure 2, and discussion of weak density in the scanning complex: It is unclear why it could be "possible that the nascent chain is present at lower occupancy" considering that the RNCs are purified by the 3x-FLAG tag on the nascent chain.
We then obtain the weak sequential density of smooth maps in W1/2(Bn,Y) and a criterion for strong density of smooth maps.
