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Further study has revealed that weakened interaction between CD44 and hyaluronan decreases the production of MCPs and consequently undermines the recruitment of mononuclear cells [21].
This result is consistent with the notion that the lpp-disrupted strain (i.e. JW1667) interacts weaker with agar, in comparison with the parental strain (i.e. BW25113), and this weakened interaction could be restored and perhaps strengthened by appropriate complementation with the Lpp expression.
In cultured cells, this weakened interaction potentiated miR-608-mediated suppression of other targets, including CDC42 and interleukin-6 (IL6).
Some solutions have been proposed in the literature to engineer better surfactants with substantially weakened interaction with proteins.
The weakened interaction between the two domains in Y279C results in an increased level of binding between the N-SH2 domain and its pTyr ligands.
Increasing the net charge causes weakened interaction between the APS2– and SAC complex and as a consequence, reduces APS channeling efficiency (Fig. 3).
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To engineer controlled degradation, we have designed a series of modified ssrA tags that have weakened interactions with ClpXP.
These weakened interactions led to a lowering of the Cu particle embedment, coinciding with a pronounced Cu crystallite growth during reduction.
The CA-NC tubular complexes that are formed with mutations in CA that help HIV-1 escape from Mx2 restriction exhibit weakened interactions with Mx2 (Fricke et al., 2014).
We conclude that the C-terminus is not necessary for CKIε to bind to substrates (see Fig. 3), but that it stabilizes otherwise weakened interactions between xDsh or mPer1 and CKIε.
Reactions employing Fis mutants that have weakened interactions with Hin (Merickel and Johnson, 2004), or Hin DBD mutants that have weakened interactions with Fis lead to processive recombination.
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