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Sporadic staining of positive stromal cells, including endothelial cells, was localised to the cytoplasm and ranged in intensity from weak to intense.
The epithelial staining of SR-B1 and HMG-CoA reductase was more heterogeneous, ranging from weak to intense (Table 2).
MIC1 expression was considered positive if more than 25% of cells showed weak to intense staining within each cylinder.
Both intratumoral and intrastromal FOXP3+CD3+ cells were present in the patients tissues and the expression level of FOXP3 in the intratumoral Tregs ranged from weak to intense.
In contrast to the bHPA method, iHPA showed heterogenous weak to intense (+++; PTs) or weak to moderate labelling of MeWo cells.
As previously observed calpastatin, calpain-1 and calpain-2 demonstrated cytoplasmic staining with some granularity and heterogeneity between adjacent tumour cells, varying from weak to intense staining.
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Additionally, the intensity of positive staining from weak to very intense staining was recorded.
The intensity of positive staining from no staining (0), weak to very intense staining was recorded.
In head & neck and colorectal cancers HIF-1α staining was less intense (weak to moderate intensity) and involved in smaller areas.
In the crypt of Lieberkühn lateral cell membranes showed weak, moderate or intense staining, the cytosol showed weak to moderate staining.
The experiments were performed for three turbulence intensities of about 0.02, 0.06, and 0.17, corresponding to weak, moderate, and intense turbulence conditions, respectively.
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