One HIV-1+2 sample (288/97) which showed strong reactivity to B6Fabenv1C and weak reactivity to B6Fabenv2C was reactive to all double antigen constructs and showed additive reactivity.
Three of the HIV-1 positive samples (C-91, C-92, C-93) showed very weak reactivity with B6Fabenv12SS and B6Fabenv12SC but were strongly reactive to two other proteins, B6Fabenv12CS and B6Fabenv12CC carrying cysteine residues in env1 sequence.
Weakly reactive sera by ANA-IIF and ELISA also showed very weak reactivity by WB, which was visualized only after film overexposure to ECL reagent.
Oxygen mustards are not as reactive as the nitrogen mustards by virtue of the weak reactivity of the oxygen atom in neutral alcoholic materials.
In the DM group, 31 of 71 patients (43.7%) showed the NFS pattern and of these, 11 sera showed weak reactivity at 1 160 dilution.
In the RA group, 24 of 47 patients (51.1%) exhibited this pattern, although the majority of these (n = 14) showed very weak reactivity at 1 160 dilution.
The 5 OB sera and 2 of the 3 HI sera producing this NFS pattern showed weak reactivity at 1 160 dilution.
Whereas, due to the weak reactivity, the magic-size cluster was observed at 180°C, indicated by the appearance of an absorption peak with a wavelength of almost 320 nm.
The serum with the weakest positive ELISA reactivity (RA-880) also showed very weak reactivity by ANA-IIF and did not produce a clearly defined DFS pattern at 1 80 or 160 dilutions (Table 3 and data not shown).
The BODIPY-based probe displayed highly sensitive and selective fluorescence enhancement respond to H2S over competing biothiols such as cysteine (Cys) and glutathione (GSH), mainly due to the weak reactivity of biothiols toward the probe.
Comparison of the own conformation of the cyclic analogues with their either strong or weak reactivity with the antibodies reveals structural features that may be correlated with the antibody reactivity.
