These ITS regions are selected for qPCR assays due to their good conservation and their weak level of polymorphism amongst DNA sequences of the same genus (Chemidlin Pretost-Boure et al. 2011; Costa et al. 2001; Iwen et al. 2002).
There are simply implications of having strong or weak levels of earned income.
The untreated controls displayed weak levels of fosfo-ATMSer1981 staining.
Another factor was the weaker level of hedging rates versus the American dollar.
However, analyses of Hsp90 amino acid sequences produce topologies that are different from those derived from analyses using Hsp90 DNA sequences (excluding the third codon positions), which can be attributed to a more conserved and thus weaker level of phylogenetic signal in the amino acid dataset [55].
As this could be due to weak phylogenetic signal resulting from high level of sequence conservation, we turned to nucleotide-based phylogeny (fig. 4 and supplementary S2 B, Supplementary Material online).
Previous studies have also shown that ASEs possess several features that distinguish them from CSEs, such as weaker signals at alternative splicing sites, shorter lengths, higher level of sequence conservation, longer flanking intronic sequences, involvement of repetitive elements on exonization, and greater frequency for skipping exons to preserve the reading frame.
Comparisons of sequence diversity and divergence between ARs, protein-coding (PC) exons and lncRNAs, and the associated flanking regions, show weak, but significantly lower levels of sequence diversity and divergence at lncRNAs compared with ARs.
Aside from the ordered PAS domains, many of the minima in PER2 have weak predictions of secondary structure and high levels of sequence conservation, serving as a useful guide for future biochemical studies of PER function.
Weak expression levels of intergenic sequences have been previously reported [ 35, 36] and these may cause weak detection levels of the intergenic transcripts.
