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Immunofluorescent labeling showed there was some weak labeling for Ccl2 in the young RPE/choroid but Ccl2 labeling was greatly increased in the aged RPE/choroid.
Weak labeling for ubiquitin was found on endosomal lysosomal structures in neuronal perikarya, and rarely, in endosomal-like structures linked with the spiral membrane inclusions.
AT1 receptors were mainly located at the cell surface and in the cytoplasm, although weak labeling for AT1R was occasionally observed at the nuclear level (Fig. 7a h).
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These were numerous and smaller and only had a weak labelling for desmin (Fig. 6B).
Only a few of the cells showed weak labelling for vimentin.
Labeling for neuroglian was primarily in the 25% sucrose fraction, with weaker labeling for the 30−35% interface and the 40+60% fraction (not shown).
In contrast to the staining in the crypts, the apical region of the villi showed a weaker labeling for PLEKHA7, whereas the labeling for ZO-1 was of similar intensity throughout the villus (arrowheads in Fig. 3F-F").
In "Multi-Instance Multilabel Learning with Weak-Label for Predicting Protein Function in Electricigens," J.-S.
Plasma membranes displayed weak labeling only (data not shown).
For CK5/6 and EGFR, sections were also evaluated for labeling intensity and location and estimated percentage of positive cells, with labeling intensity recorded as 0 (no labeling), 1+ (weak labeling), 2+ (moderate labeling), or 3+ (strong, intense labeling) [ 4].
Labeling intensity was recorded as 0 (no labeling), 1+ (weak labeling above background), 2+ (moderate labeling), or 3+ (strong labeling) as previously described [ 17].
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