Using the stable fluorescent properties of NP1 we have previously observed a robust internalization into mesenchymal-derived cells such as osteoblast, however, a much weaker internalization into cells from other lineages such as epithelial.
To test whether the reduction in the levels of cholesterol in these older cells might be responsible for the weaker receptor internalization of 30 DIV neurons, these cells were incubated with the cholesterol-MβCD (30 μM) and cholesterol (5 μM) replenishment mix.
Our analysis explains how imposing weaker assumptions on the internalization machinery, namely that the two pathways are entered with distinct affinities, is sufficient to explain the observed switch-effect.
For females but not males, social comparison and internalization acted as weak but significant mediators between sociocultural influence and body image concerns.
Similarly, the assumption that ADCC and CDC contribute to the anti-tumour activity of ADCs [ 104, 105] might prove to be incorrect as these effector functions are generally weak and compete with ADC internalization.
Molecules lacking one of these interactions showed other modes of action resulting in weaker binding or dramatically reduced internalization rates [31].
In this context, common to Continental and Mediterranean countries, the discipline effect of competition or the internalization of externalities are weak.
The weak influence of wild-type β2AR on internalization of β2AR D113S-Rluc8 prompted us repeat the same basic experiment using a more conventional method of detecting internalization.
Transplanted organs are populated by immature DC that are phagocytic with high capability for Ag internalization and processing, but that exhibit weak stimulatory capacity for naïve T cells due to the low levels of Ag-presenting and costimulatory molecules on the DC surface.
