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We detected weak expressions of CTIP2 in primary human epidermal keratinocytes (Hek-a) and in spontaneously immortalized non-tumorigenic human skin keratinocytes (Hacat) [Fig. 2E].
However, weak expressions were detected in A549, H1299 and H292 cell lines compared with SCLC cells.
In corresponding normal muscle and colon tissues, there were very weak expressions of PIG-2.
And weak expressions are also detected in the posterior proximal limb buds (n = 2/4.
However, ER β nuclear strong expression (n = 39) contributes by 19 ER β cytosol strong expressions and 20 weak expressions.
The strong, medium and weak expressions in IHC were defined as positive staining and, in contrast, the absent expression as negative staining.
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RT-PCR showed that PiTRAF3 was expressed in all tissues, with relatively weak expression in the heart and liver.
By contrast the ECB's own summer hit-about sounds less like a global sporting juggernaut, more like a slightly weak expression of exasperation.
There was weak expression in heart ventricles.
This is due to the weak expression of PIs.
HRPE, human primary RPE cells; -, no expression; +, weak expression; +++, strong expression.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com