Interestingly, the pattern of Armadillo staining did not change during infection in flies depleted of EGFR in enterocytes, but instead a weak and constant Armadillo staining was observed in enterocytes.
However, the critical temperature Tc is often lower than that expected from the weak coupling constant and a given Δ as an effect of strong coupling.
As hole concentration decreases from overdoped to underdoped Bi2212, the experimental data point moves apart from the weak coupling point toward the strong coupling side, and a crossover occurs at 8.5, which is about twice the weak coupling constant.
During the minimization, heating and equilibration all atoms of the complex were restrained to their initial positions by a weak force constant of 2 kcal mol−1 * Å−2.
These interfaces should conduct muscle contraction force, but not to damage the long nerves involved, elastic nature of the interface should make tensile stress weak and constant.
Unexpectedly, SPR experiments carried out with XcOGT revealed a weak binding constant of alloxan to XcOGT (Kd > 1 mM).
It was necessary to use the higher salt conditions to compensate for the weak homodimerization constant of p51.
Presumably reflecting the weak binding constant and the lattice packing, we have been unable to obtain crystals of SNF1 with nucleotide present in the weaker site.
Further, the relatively weak inhibition constant (IC50 of 238 μM or 71 μg/mL) was consistent with the partial phenotypes noted in our phenotypic and genetic assays.
Based on theoretical scattering curves (CRYSOL) for the monomer and dimer (PDB: 1FTJ), the fraction of dimer present was near zero for the GluA2 LBD, consistent with its weak association constant, and 0.99 for the nondesensitizing L483Y mutant.
The ParM variant, labeled with two tetramethylrhodamines, binds ADP with relatively weak affinity (dissociation constant 30 μM) but responds to ADP binding with ∼15-fold signal increase.
