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At moderate pressures, weak amplification is predicted since flame temperature decreases and recombination reaction dominates.
Nine other candidate genes AK5, ACVRL1, AMHR2, CERKL, MAFA, MAGI2, PIP5K1B, FAM49A, and TPRXL showed weak amplification.
By direct PCR, we only detected weak amplification bands in some samples, in agreement with the presence of low biomass even in altered samples.
Very weak amplification products were obtained from three subjects with the lowest bacterial copy numbers and we did not identify bacterial sequences from these libraries.
Due to the comparatively slow and weak amplification of P2 vir1 on Y. pestis, this phage was excluded from further experiments.
Weak amplification bands were obtained for 4.3 m, 66.2 m and 68.0 m levels, but in too low amount to construct SSU rDNA libraries for molecular diversity purposes.
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We observed weak amplifications in 16 samples (11%); all were from P. vivax infected blood.
Weak amplifications in PD-PCR may be attributed to the fact that the actual amplification in conventional PCR is one step ahead than the PD-PCR.
In a few cases, weak amplifications of the RPB1 gene were used as a template for a second PCR with the same primer combination.
Of these, 61 (63.5%) primer pairs produced repeatable and reliable amplifications in at least four accessions of tea, while 35 (36.5%) primer pairs either completely failed or led to weak amplifications and thus were excluded from further analysis.
Of the 707 EST-SSR primer pairs, 691 (97.74%) produced repeatable and reliable amplifications of expected size in at least one line of the five B. rapa parental lines (Chiifu 401, Kenshin, Rapid cycling B. rapa, 94 SK and CR Shiki) screened, while 16 (2.26%) primer pairs either completely failed or led to weak amplifications and thus were excluded from further analysis.
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