This result is consistent with the notion that the lpp-disrupted strain (i.e. JW1667) interacts weaker with agar, in comparison with the parental strain (i.e. BW25113), and this weakened interaction could be restored and perhaps strengthened by appropriate complementation with the Lpp expression.
C. protea was grown on weak malt yeast agar plates (0.02 g Yeast extract, 0.02 g Malt extract, 0.75 g K2HPO4, 1 L ddH2O, 15 g Agar) with streaks of Escherichia coli as food.
The Δ crp mutant of this biovar only lost the ability to decarboxylate lysine, ferment L-arabinose and produce H2S in triple sugar iron agar (weak reaction in the wild type strain).
Samples considered weak positive to the agar gel immunodiffusion (AGID) test were "true" positive in the ELISA.
Protosteloid amoebae were grown on weak malt yeast extract agar plates (wMY) (0.002 g malt extract, 0.002 g yeast extract, 0.75 g K2HP04, 15.0 g Difco Bacto Agar, 1.0L deionized [DI] H20) with appropriate food organisms (Table 3) in the laboratory at ambient temperatures (approx 21 25°C) [2], [3].
They grow as dimorphic gray colonies <1 mm in diameter, are nonmotile and non spore forming, and show <span class="lh">weak β-hemolysis on agar plates containing 5% horse or sheep blood after 3 5 days of growth.
As had been previously reported for a number of the proteins tested in this screen, the infection of MCF10A cells with single viral vectors led to no or very weak growth in soft agar (data not shown).
Actinobaculum schaalii grows slowly after 48 h in an anaerobic atmosphere at 37°C as tiny grey colonies, less than 1 mm in diameter, and shows weak β-hemolysis on agar plates containing 5% horse or sheep blood.
Growth on the acetamide agars was weak for all strains and it was often difficult to determine whether true growth had occurred.
Cell cultures in early exponential growth phase, containing about 80 µg total chlorophyll, were harvested by weak centrifugation and layered on TAP agar medium in the instrument containers.
