Segregants at a locus for which both parents have a double dose will segregate in a ratio of 35:1, and segregants at a locus for which one parent has a single dose and the other a double dose will segregate in a ratio of 11 1, even though the primer pairs amplify a product from both parents and therefore do not appear to detect a polymorphism.
Therefore, we can assume that nearly all of these mutations will segregate independently, and the likelihood of having tightly linked, off-target functional mutations that confound phenotype interpretation will be rare.
Linked copies will segregate as a single copy and will therefore not be noticed in Mendelian segregation studies.
If there is no preferential pairing between homeologous pairs of chromosomes, segregants at a locus for which both parents have a single dose will segregate in a ratio of approximately 3 1.
The purpose was to make use of immiscibility between Co and Cu, hoping that Cu will segregate at Co-grain-boundaries to mediate magnetic decoupling.
Later, supersaturated In in Cu–In compound will segregate from the liquid droplets to interact with oxygen and to form In2O3 solid.
If the base of the lithosphere happens to exist just above this depth, then any further mantle upwelling and melting will be prevented, and the near-solidus melt will segregate and ascend into the lithosphere.
If two adjacent markers are more distant from each other than the size of the BAC clones (∼80 Kbp), they will segregate independently.
As any mutation occurring in the library will segregate in a Mendelian fashion in the M2 offspring, pooling of leaf material of five individual plants reduces the likelihood of not sampling a mutation as consequence of segregation to 0.1% (0.510 = 0.001).
While re-sequencing studies can detect large numbers of singleton SNPs in many population samples (depending on population history), the selection of informative SNPs for genotyping studies (i.e., ascertainment) ensures that most SNP marker alleles will segregate at intermediate frequency, exaggerating the difference in frequency spectrum that already exists between SNPs and SSRs [3], [4].
