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Nevertheless, most SOD1 fusion proteins were stable when expressed in PC12 cells, although a subset were cleaved; we note that under experimental conditions to detect GFP, free GFP or truncated GFP-SOD1 fusion proteins may be detected, rather than intact SOD1-GFP fusion proteins.
The His-tag (Tat) and His-GB1-tag (AFF) were cleaved with TEV protease.
Coral colonies were cleaved in half.
Only small amounts of transcripts were cleaved.
These constructs were cleaved with AgeI and SphI.
Peptides were cleaved from the resins with a TFA/H20/thioanisole/ethanedithiol/phenol mixture.
A few nuclei were cleaved.
Cytoskeletal filaments were cleaved and formed clusters.
The tags were cleaved off by thrombin.
The Rh S bonds were cleaved concurrently.
The relative number of PKs that were cleaved was ∼14%.
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