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The degree of FOXO6 knockdown in Figure 3C at the protein level is 50% (despite 80-90% knockdown at the mRNA level), which may be due technical issues related to the antibodies used (see Discussion about ChIP for FOXO6 above), or to the relatively long half-life of the protein (we waited up to 96 hours after siRNA transfection to assay for knockdown).
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"If folks waited up, we certainly appreciate them and love their passion for wanting to buy a product from Amazon".
Valerie James waited up for O'Neill.
She waited up for him at night.
"We'd wait up for Dad after his.
"Sometimes we have to wait up to one hour at the checkpoint while we see settlers driving through it without even stopping," she added.
To get back to the America of the national dream will take a long walk – so long we shouldn't wait up for Bruce.
Then we waited, hopped up and buzzing.
Arriving without a reservation on a Saturday night (they are accepted for only half the tables), our party of eight was told by the chipper hostess that we could expect to wait up to two hours.
"We have people that wait up at night for the counter price to change.
"They're probably all waiting up".
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com