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We verified the changes observed in lipid metabolism proteins by measuring Apo-AI and Apo-B concentrations (representing HDL-cholesterol and LDL-cholesterol respectively) by ELISA in a larger group of samples collected from malaria-infected and uninfected primigravidae and multigravidae with and without SA (primigravidae n = 150; multigravidae n = 145).
Based on the reverse transcription polymerase chain reaction (RT PCR) and real-time PCR analyses, we verified the changes in expression levels for a number of candidate genes.
We verified the changes in the expression patterns of some of these miRNAs using quantitative real-time PCR (qPCR; Figure 4).
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Semantic schema generator once the change is found to be plausible, we verify the changes in a semantic schema generator rather than implementing the changes directly in the service level.
We verified the expression changes of the only up-regulated miRNA, miR-21: in the qPCR assays, it was 3.5 fold up-regulated in the kidney of diseased animals, compared to healthy ones.
Furthermore, we also verified the change in the EMT process by phalloidin-labeled staining to visualize cytoskeletal actin expression changes in MCs.
Next, we verified the early-age-related changes in activity of neutrophil marker, myeloperoxidase (MPO) based on the recent reports demonstrating the higher MPO activity in the serum of elderly humans [42] and rat kidney's [43].
Data from the MacConkey plates verified the changes in carbohydrate metabolism observed in the Biolog™ system.
The analysis verified the changes in the mRNA profiling in differentiating vs. proliferating cells (Fig. 1a c).
The simulation results verified the change of the semiconductor characteristic and resistance before and after hydrogen interaction.
We verified that changes in mRNA expression in KO BAT correlated with protein levels.
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CEO of Professional Science Editing for Scientists @ prosciediting.com